FOR THE MEDICAL PRACTITIONER
Dermatovenereology
doi: 10.25005/2074-0581-2026-28-1-190-205
THE TRANSCRIPTOME PROFILE OF PATIENTS WITH SEVERE PSORIASIS

N.D. ZDZITOVETSKAYA1, T.G. RUKSHA1, YU.V. KARACHYOVA1, V.O. KOBANENKO1, E.I. BONDAR2,3

1Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky, Krasnoyarsk, Russian Federation
2Laboratory of Genomic Research and Biotechnology, Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
3School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation

Objective: This study aimed to identify differentially expressed genes in the skin of psoriasis patients with MTX resistance using transcriptome profiling to evaluate the molecular basis of treatment efficacy.

Methods: The study included 6 patients with widespread psoriasis vulgaris receiving MTX (7.5-25 mg/week) combined with folic acid (5 mg/week). Patients were categorized into two groups based on their 6-month clinical response: responders and non-responders. A control group consisted of patients with widespread psoriasis who were MTX-naïve. Skin biopsies were collected from lesional skin, preserved in RNA-stabilizing solution, and processed for total RNA extraction.

Results: Genetic analysis identified key molecular markers associated with chronic inflammatory disease progression and the development of drug resistance.

Conclusion: Individual transcriptomic profiling in patients with severe psoriasis may facilitate the development of personalized therapeutic approaches.

Keywords: Psoriasis, methotrexate, transcriptome profile, resistance, total RNA.

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Authors' information:

Zdzitovetskaya Natalia Dmitrievna,
Assistant Professor of the Department of Dermatovenereology named after Professor V.I. Prokhorenkov with a Course in Cosmetology and PE, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky
ORCID ID: 0000-0002-2976-8748
SPIN: 7685-4887
E-mail: nzdzit100195@gmail.com

Ruksha Tatyana Gennadievna,
Doctor of Medical Sciences, Full Professor, Head of the Department of Pathological Physiology named after Professor V.V. Ivanov, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky
ORCID ID: 0000-0001-8142-4283
SPIN: 5412-2148
E-mail: tatyana_ruksha@mail.ru

Karachyova Yuuliya Viktorovna,
Doctor of Medical Sciences, Associate Professor, Head of the Department of Dermatovenereology named after Professor V.I. Prokhorenkov with a Course in Cosmetology and PE, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky
ORCID ID: 0000-0003-2619-9786
SPIN: 4789-9178
E-mail: julkar19@yandex.ru

Kobanenko Vladislav Olegovich,
Junior Researcher at the Laboratory of Medical Cybernetics and Healthcare Management, Lecturer at the Department of Medical Cybernetics and Informatics, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky
ORCID ID: 0000-0003-3889-1956
SPIN: 1143-4417
E-mail: kobanenko1999@bk.ru

Bondar Evgeniya Ivanovna,
Researcher, Laboratory of Genomic Research and Biotechnology, Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences, Senior Lecturer of School of Fundamental Biology and Biotechnology, Siberian Federal University
ORCID ID: 0000-0003-3762-6974
Е-mail: bondar.zhenya.iv@gmail.com

Information about support in the form of grants, equipment, medications

This research was conducted as part of a doctoral dissertation (Candidate of Medical Sciences). The study was supported by an internal grant from the Krasnoyarsk State Medical University, named after Professor V.F. Voyno-Yasenetsky (Order No. 533, dated October 7, 2022). The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:

Zdzitovetskaya Natalia Dmitrievna
Assistant Professor of the Department of Dermatovenereology named after Professor V.I. Prokhorenkov with a Course in Cosmetology and PE, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky

660022, Russian Federation, Krasnoyarsk, Partizana Zheleznyaka str.,1

Tel.: +7 (913) 5697490

E-mail: nzdzit100195@gmail.com


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