Pharmacology
doi: 10.25005/2074-0581-2019-21-3-450-454
ANTI-TUMOR ACTIVITY OF DRY EXTRACT PADUS GRAYANAE MAXIM IN VITRO

I.Z. Ismailov, A.Z. Zurdinov, T.S. Sabirova

Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy, Bishkek, Kyrgyz Republic

Objective: To study the antitumor activity of dry extract Padus Grayanae Maxim in vitro conditions.

Methods: The object of the study was dry extract Padus Grayanae Maxim, obtained by lyophilic drying method from water-alcohol extraction of plant medicinal raw materials and standardized in content chlorogenic and coffee acids. The study of the cytotoxicity of Padus Grayanae Maxim dry extract conducted on cultures of tumor cells that were derived from the American Type Culture Collection (ATCC). Cell mortality was assessed using the MTT test to form a non-soluble formazan.

Results: It has been established that the studied phytoextraction an antitumor effect in relation to the lines of tumor cells of human cervical adenocarcinoma (HeLa), stomach adenocarcinoma (AGS), human pancreatic carcinoma (MiaPaCa2) and rhabdomyosarcoma of mice (RD). At the same time, fairly high specificity of the action of the studied phytoextraction revealed in relation to the tumor cells adenocarcinoma of the human stomach (AGS). The cell line of tumor cells of human hepatocellular carcinoma (HepG2) has shown resistance to dry extract Padus Grayanae Maxim.

Conclusion: The antitumor effect of the dry extract Padus Grayanae Maxim in vitro conditions has been revealed. At the same time, a specific action against various tumor strains may be due to flavonoids, chlorogenic and coffee acids, which have a wide range of cytotoxicity.

Keywords: Dry extract Padus Grayanae Maxim, antitumor activity, flavonoids, chlorogenic acid, coffee acid.

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Authors' information:

Ismailov Isabek Zaylidinovich
Doctor of Pharmaceutical Sciences, Associate Professor, Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy
ORCID ID: 0000-0002-1468-8251
Author ID: 877010
SPIN: 9540-1750
E-mail: ism-isa@mail.ru

Zurdinov Ashirali Zurdinovich
Doctor of Medical Sciences, Professor of the Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy
ORCID ID: 0000-0002-7110-0901
Author ID: 877038
SPIN: 3719-0291
E-mail: a-zurdinov@yandex.ru

Sabirova Tamara Semyonovna
Candidate of Medical Sciences, Associate Professor, Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academy
ORCID ID: 0000-0002-3709-0244
Author ID: 877003
SPIN: 1684-5129
E-mail: sabirova_ts@mail.ru

Information about support in the form of grants, equipment, medications

The work was carried out according to the plan of scientific research works of I.K. Akhunbaev Kyrgyz State Medical Academy (state registration number – 0004828). The authors did not receive financial support from manufacturers of medicines and medical equipment.

Conflicts of interest: No conflict

Address for correspondence:

Ismailov Isabek Zaylidinovich
Doctor of Pharmaceutical Sciences, Associate Professor, Department of Basic and Clinical Pharmacology, I.K. Akhunbaev Kyrgyz State Medical Academ

720020, Kyrgyz Republic, Bishkek, Akhunbaev Street, 92

Tel.: +996 (559) 639 777

E-mail: ism-isa@mail.ru

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