Dentistry

doi: 10.25005/2074-0581-2021-23-4-532-541
ELECTRON MICROSCOPIC PROPERTIES OF A NEW ANTIMICROBIAL NANODRUG BASED ON COPPER-CALCIUM HYDROXIDE COMPOUND

V.A. Rumyantsev1, G.A. Frolov2, A.V. Blinova1, Ya .N. Karasenkov3, E.V. Bityukova1

1Department of Periodontology, Tver State Medical University, Tver, Russian Federation
2Department of Physical Chemistry, National University of Science and Technology "MIS iS", Moscow, Russian Federation
3"RosDent" Medical Company, Moscow, Russian Federation

Objective: To study the structural arrangement of the copper-calcium hydroxide (CCH), a commercially available dental paste used in aqueous suspension, as well as a new antimicrobial nanodrug based on CCH and a hydrosol of copper nanoparticles and copper (II) oxide.

Methods: Dental paste Cupral® manufactured by "Humanchemie GmbH" (Germany) was investigated. Immediately prior to the study, it was diluted with either distilled water or a hydrosol of copper nanoparticles and copper (II) oxide in a ratio of 1:2. The hydrosol was obtained in the Department of Physical Chemistry laboratory at the National University of Science and Technology "MISiS", using the method of condensation of low-temperature plasma in a spark discharge. The microstructure of the obtained samples was analyzed using a transmission electron microscope (TEM), LEO 912 AB OMEGA (Carl Zeiss, Oberkochen, Germany).

Results: Dilution of CCH with a hydrosol of copper nanoparticles and copper (II) oxide results in forming a complex nanosized composite structure. Nanoparticles in the dispersed phase penetrate the surface layers of CCH particles, which are crystalline hydrates of copper (II) oxide and calcium oxide.

Conclusion: The discovered phenomenon could be helpful in the design of a new technology of nanoimpregnation of the tooth root dentin in endodontic and periodontal treatment, which does not require additional electrophoresis.

Keywords: Endodontics, periodontology, nanotechnology, copper-calcium hydroxide, electron microscopy.

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Authors informations:

Rumyantsev Vitaliy Anatolievich
Doctor of Medical Sciences, Full Professor, Head of the Department of Periodontology, Tver State Medical University
ORCID ID: 0000-0001-6045-3333
SPIN: 1922-4850
Author ID: 627641
E-mail: rumyancev_v@tvergma.ru

Frolov Georgiy Aleksandrovich
Candidate of Chemical Sciences, Associate Professor of the Department of Physical Chemistry, National University of Science and Technology "MISiS"
ORCID ID: 0000-0003-1460-6030
E-mail: georgifroloff@yandex.ru

Blinova Alisa Vladimirovna
Postgraduate Student of the Department of Periodontology, Tver State Medical University
ORCID ID: 0000-0002-4315-163X
SPIN: 4239-0519
Author ID: 985695
E-mail: blinova-alisa@mail.ru

Karasenkov Yakov Nikolaevich
Candidate of Medical Sciences, Chief Medical Officer of the "RosDent" Medical Company
ORCID ID: 0000-0002-9658-3700
E-mail: rosdent@mail.ru

Bityukova Elena Vladimirovna
Candidate of Medical Sciences, Associate Professor of the Department of Periodontology, Tver State Medical University
ORCID ID: 0000-0002-6225-8344
SPIN: 4004-4569
Author ID: 1032251
E-mail: bitykova_l@mail.ru

Information about the source of support in the form of grants, equipment, and drugs

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflict of interest: No conflict

Address for correspondence:

Blinova Alisa Vladimirovna
Postgraduate Student of the Department of Periodontology, Tver State Medical University

170100, Russian Federation, Tver, Sovetskaya str., 4

Tel.: +7 (919) 0516059

E-mail: blinova-alisa@mail.ru

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