ORIGINAL RESEARCH

Pathology

doi: 10.25005/2074-0581-2023-25-4-466-477
THE ROLE OF SCHWANN CELLS IN THE PATHOLOGY OF THE HUMAN APPENDIX IN CHILDREN

M. KAPITONOVA1, I.B. BROHI1, S. GUPALO2, A.V. SMIRNOV3, V.S. PETRENYUK4, A. AHMAD5

1Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan, Malaysia
2Saint James School of Medicine, Valley, Anguilla
3Volgograd State Medical University, Volgograd, Russian Federation
4Municipal Clinical Emergency Hospital № 7, Volgograd, Russian Federation
5Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Malaysia

Objective: To compare the immunohistochemical (IHC) characteristics of appendices removed from children with acute appendicitis and chronic pain syndrome (CP) in the right lower quadrant of the abdomen (RLQA), as well as to define clinical and morphological correlations.

Methods: The structure of fifty-one appendices of children aged 5-14 years who underwent appendectomy for chronic appendicitis/CP in the RLQA (24 patients, Group 1) and acute appendicitis (27 patients, Group 2) was assessed. Image analysis of histological sections stained with hematoxylin-eosin and immunohistochemically for S100 protein and proliferating cell nuclear antigen (PCNA) was performed.

Results: Image analysis showed the presence of a significantly higher volume density (VD) and numerical density (ND) (p<0.05) of immunoreactive cells when stained for S100 protein, as well as the ND of intramural ganglia (p<0.05) against the background of minimal histological changes in the wall of the appendix in patients of Group 1 compared to Group 2. On the contrary, the ND of PCNA-immunoreactive cells was significantly higher in patients of Group 2 against the background of pronounced inflammatory changes in the appendix (p<0.01). In the biopsy samples of the Group 1 patients, a significantly higher ND of the lymphoid nodule germinal centers was also noted (p<0.05), while the ND of tingible body macrophages was higher in Group 2 patients (p<0.001). These results demonstrate differences in neuroimmune parameters in the appendices in the two described nosologic groups and indicate the possible role of the appendiceal neural component as a prerequisite for developing CP in the RLQA in children

Keywords: Appendix, Schwann cell, S100 protein, PCNA, appendicitis, chronic abdominal syndrome.

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Author information:


Kapitonova Marina,
MD, PhD, Professor, Professor of Anatomy, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak

Researcher ID: Y-6429-2018
Scopus ID: 8854275100
ORCID ID: 0000-0001-6055-3123
SPIN: 5647-3218
Author ID: 121504
E-mail: kmarina@unimas.my

Brohi Imam Bux,
MD, Associate Professor of the Family Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak
ORCID ID: 0000-0001-7574-2154
E-mail: bimam@unimas.my

Gupalo Sergey,
MD, MS, Associate Professor of Pathology, Saint James School of Medicine
Scopus ID: 56657520000
ORCID ID: 0000-0002-8487-1409
E-mail: sgupalo@mail.sjsm.org

Smirnov Aleksey Vladimirovich,
MD, PhD, Professor, Head of Pathology Department, Volgograd State Medical University
Scopus ID: 57213379797
ORCID ID: 0000- 0001-5351-6105
E-mail: alexey-smirnov@rambler.ru

Petrenyuk Vyacheslav Savvovich,
MD, General Surgeon, Head of the 1st Children’s Surgical Department, Municipal Clinical Emergency Hospital № 7 (died in May, 2023)
Scopus ID: 6505949589

Ahmad Azhar,
BSc, MSc, Researcher, Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA
Scopus ID: 57212513419
ORCID ID: 0000- 0001-5456-079X
E-mail: biotech.azhar@gmail.com

Information about support in the form of grants, equipment, medications

The study was supported by grant of the Malaysian Ministry of Higher Education FRGS/2/2013/SKK01/UITM/01/4/. The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:


Gupalo Sergey,
MD, MS Associate Professor of Pathology, Saint James School of Medicine

A-1, Albert Lake Dr, The Quarter 2640, Anguilla

Tel.: +6017 6205710

E-mail: sgupalo@mail.sjsm.org

Materials on the topic: