Pediatrics
doi: 10.25005/2074-0581-2023-25-2-192-200
OXIDANT AND ANTIOXIDANT STATUS IN NEONATES WITH SPECIFIC INTRAUTERINE INFECTIONS

K.I. Ismoilov1, Sh.S. Muzaffarov1, A.M. Saburova2

1Department of Pediatric Diseases № 2, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan
2Department of Biochemistry, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikista

Objective: To evaluate lipid peroxidation (LPO) and antioxidant defense (AOD) system in full-term and preterm neonates with specific intrauterine infections (IUI).

Methods: Eighty full-term and preterm newborns with specific IUI were examined; 48 of them (60%) with a severe course of IUI, and 32 (40%) with an extremely severe IUI course. The control group included 30 relatively healthy newborns, including 22 full-term and 8 late premature (born at 34-37 weeks of gestation) neonates. The state of LPO and AOD was assessed by the levels of malondialdehyde (MDA), superoxide dismutase (SOD), ascorbic (AA), and sialic (SA) acids. Enzyme-linked immunosorbent assay (ELISA) of blood serum of newborns with IUI and their mothers was carried out in paired sera, with IgG, IgM, and avidity level (%) of IUI pathogens determined.

Results: Analysis of epidemiological data on TORCH infection in the examined neonates revealed diagnostic titers of cytomegalovirus infection (91.3%), herpes (70.4%), toxoplasmosis (50.1%), and chlamydia (43.4%). Comparative analysis of MDA level in the first and control groups showed a statistically significant difference (p<0.05); while its comparison between the 2nd and control group showed even higher level difference (p<0.05). The levels of SOD, AA and SA in the 1st and 2nd groups were highly significantly different from the control group (p<0.001). All these tests showed significant differences between the 1st and the 2nd group (p<0.05), except for the levels of sialic acid (p>0.05).

Conclusion: In neonates with specific IUI, statistically significant changes in LPO and AOD parameters were obtained compared with the control group. Enzymatic and non-enzymatic antioxidant parameters can be diagnostically significant for early prediction of infectious processes in the body of a newborn. The revealed changes in the LPO and AOD indicators in the neonates with IUI, dictate the need for timely and adequate antioxidant therapy along with etiotropic treatment.

Keywords: Homeostasis, lipid peroxidation, intrauterine infections, antioxidant defense, tricarboxylic acid cycle.

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Authors' information:

Ismoilov Komildzhon Isroilovich,
Doctor of Medical Sciences, Full Professor, Professor of the Department of Pediatric Diseases № 2, Avicenna Tajik State Medical University
ORCID ID: 0000-0003-2431-1551
SPIN: 6886-8004
Author ID: 847074
E-mail: ismoilov52@inbox.ru

Muzaffarov Shamshod Sabokhiddinovich,
Competitor of the Department of Pediatric Diseases № 2, Avicenna Tajik State Medical University
ORCID ID: 0000-0002-5719-1114
SPIN: 2992-4034
Author ID: 1112803
E-mail: sha.zavr@mail.ru

Saburova Anna Mukhammadievna,
Doctor of Biological Sciences, Full Professor, Professor of the Department of Biochemistry, Avicenna Tajik State Medical University
ORCID ID: 0000-0002-5719-1114
SPIN: 9132-9748
Author ID: 425954
E-mail: 20@tajmedun.tj

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:

Muzaffarov Shamshod Sabokhiddinovich
Competitor of the Department of Pediatric Diseases № 2, Avicenna Tajik State Medical University

734003, Republic of Tajikistan, Dushanbe, Rudaki Ave., 139

Tel.: +992 (939) 307344

E-mail: sha.zavr@mail.ru

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