MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS
1Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russian Federation
2Irkutsk State Medical Academy of Postgraduate Education – Branch Campus of the Russian Medical Academy of Continuing Professional Education, Irkutsk, Russian Federation
Objective: To identify the morphological patterns of development and prevention of experimental epidural fibrosis based on the shape of the dural sac in the area of laminectomy.
Methods: Male Wistar rats aged 4-5 months underwent laminectomy at the level of L6-S1. The animals were divided into two groups with 7 animals per group. In the main group (Group A) the animals were treated with Antiadhesin® gel applied to the wound to prevent the development of epidural fibrosis, while in the group of comparison (Group B), the gel was not applied. Another 7 healthy animals were used as a control group. The animals were taken out of experiment on the 28th day. Histological slides stained with hematoxylin-eosin were used to measure the width and length of the dural sac, its area, perimeter, and calculate its deformity index.
Results: In group B at the level of laminectomy, the ratio of the width to length of the dural sac was significantly higher than in the control group (p<0.05), mainly due to the enlarged width, while the length did not change significantly. In group A these parameters did not differ from the control group, which indicated that application of Antiadhesin® prevented formation of a connective tissue scar which could compress the dural sac and cause its deformation. In group A in the area of surgical intervention, granulomas with Pirogov-Langhans cells were detected, both in the soft tissues and in the lumen of the spinal canal. These findings in perioperative tissues might be related to biocompatibility and indicate their reaction to a gel as a foreign body.
Conclusion: In clinical practice optimal materials for the prevention of epidural fibrosis are missing. There is an obvious need for further research in order to obtain implants capable of inhibiting and reducing the formation of epidural fibrosis.
Keywords: Dura mater, epidural fibrosis, prevention, experiment, laminectomy, Antiadhesin®.
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Shurygina Irina Aleksandrovna Doctor of Medical Sciences, Full Professor, Deputy Director for Science, Irkutsk Scientific Centre of Surgery and Traumatology ORCID ID: 0000-0003-3980-050X SPIN: 6745-5426 Author ID: 161435 E-mail: email@example.com
Zhivotenko Aleksandr Petrovich Researcher, Research Clinical Department of Neurosurgery, Irkutsk Scientific Centre of Surgery and Traumatology ORCID ID: 0000-0002-4032-8575 SPIN: 8016-5626 Author ID: 959049 E-mail: firstname.lastname@example.org
Goldberg Oleg Aronovich Candidate of Medical Sciences, Leading Researcher, Irkutsk Scientific Centre of Surgery and Traumatology ORCID ID: 0000-0003-1040-284X SPIN: 1591-9935 Author ID: 559901 E-mail: email@example.com
Sorokovikov Vladimir Alekseevich Doctor of Medical Sciences, Full Professor, Director, Irkutsk Scientific Centre of Surgery and Traumatology; Head of the Department of Traumatology, Orthopedy and Neurosurgery, Irkutsk State Medical Academy of Postgraduate Education – Branch Campus of the Russian Medical Academy of Continuing Professional Education ORCID ID: 0000-0002-9008-6383 SPIN: 8379-4458 Author ID: 441270 E-mail: firstname.lastname@example.org
Larionov Sergey Nikolaevich Doctor of Medical Sciences, Leading Researcher, Head of the Scientific and Clinical Department of Neurosurgery, Irkutsk Scientific Centre of Surgery and Traumatology ORCID ID: 0000-0001-9189-3323 SPIN: 6720-4117 Author ID: 639993 E-mail: email@example.com
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Zhivotenko Aleksandr Petrovich
Researcher, Research Clinical Department of Neurosurgery, Irkutsk Scientific Centre of Surgery and Traumatology
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