General Surgery
doi: 10.25005/2074-0581-2020-22-3-455-460
ROLE OF MONOCYTES IN PATHOGENESIS OF GENERALIZED PERITONITIS

A.R. Saraev, Sh.K. Nazarov, S.G. Ali-Zade

Department of Surgical Diseases № 1, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan

Objective: To study the sepsis markers informativeness to assess the role of monocytes in the pathogenesis of generalized peritonitis (GP).

Methods: The study included 160 patients with GP, who were divided into 3 groups, according to the stages of the disease. To establish the activity of monocytes was made a determination of the level of cytokine TNF-α and presepsin in the blood.

Results: Studies showed that the level of TNF-α in patient with septic shock was reliably lower (24.5±13.3 pg/ml) than in patients with endogenous intoxication and abdominal sepsis. The value of TNF-α in deceased patients also was low – 4.8±0.9 pg/ml. This indicates a decrease in the ability of monocytes in GP at the stage of septic shock to exude a sufficient amount of pro-inflammatory cytokines in response to endotoxin aggression. The level of presepsin increased by stages and amounted to 355.6±8.6, 783.4±24.0 and 1587.7±70.5 pg/ml, respectively. This indicates the circulation in the blood of the CD14 receptor, which is able to express on monocytes, converting them into endothelial progenitor cells.

Conclusion: Monocytes as endothelial progenitor cells contribute to the regeneration and restoration of endothelial function in its dysfunction that develops in GP and abdominal sepsis. In consequence of developing immunosuppression and suppression of monocyte function in the stage of septic shock, the process of renewal of endothelial cells is weakened, the secretion of pro-inflammatory cytokines, in particular TNF-α, decreases, which can contribute to an increase in mortality in septic shock.

Keywords: Monocytes, abdominal sepsis, septic shock, endothelial dysfunction, progenitor cells.

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Authors' information:

Saraev Alisher Rakhmatulloevich
Candidate of Medical Sciences, Associate Professor of the Department of Surgical Diseases № 1, Avicenna Tajik State Medical University
Researcher ID: F-5504-2019
ORCID ID: 0000-0001-9695-1924
SPIN: 6911-9887
Author ID: 849178
E-mail: dr.saraev@mail.ru

Nazarov Shokhin Kuvvatovich
Doctor of Medical Sciences, Full Professor, Head of the Department of Surgical Diseases № 1, Avicenna Tajik State Medical University
Researcher ID: C-4033-2019
ORCID ID: 0000-0003-2099-2353
SPIN: 1817-7089
Author ID: 845034
E-mail: shohin67@mail.ru

Ali-Zade Sukhrob Gaffarovich
Candidate of Medical Sciences, Associate Professor of the Department of Surgical Diseases № 1, Avicenna Tajik State Medical University
Researcher ID: B-6807-2019
ORCID ID: 0000-0002-2456-7509
SPIN: 6854-5343
Author ID: 258253
E-mail: suhrob_a@mail.ru

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:

Saraev Alisher Rakhmatulloevich
Candidate of Medical Sciences, Associate Professor of the Department of Surgical Diseases № 1, Avicenna Tajik State Medical University

734003, Republic of Tajikistan, Dushanbe, Rudaki Ave., 139

Tel.: +992 (918) 650540

E-mail: dr.saraev@mail.ru

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