ORIGINAL RESEARCH
Endocrinology
doi: 10.25005/2074-0581-2026-28-2-445-453
MARKERS OF ENDOTHELIAL DYSFUNCTION IN CHILDREN WITH TYPE 1 DIABETES MELLITUS

YU.S. AKSYONOVA1, А.P. VOROBYOVA2, YU.V. BYKOV1,3, V.А. BATURIN1, V.V. МASSOROV2, G.A. AVAKYAN1

1Stavropol State Medical University, Stavropol, Russian Federation
2Children's City Clinical Hospital named after G.K. Filippsky, Stavropol, Russian Federation
3Regional Children's Clinical Hospital, Stavropol, Russian Federation

Objective: To assess the serum levels of markers of endothelial glycocalyx (EG) degradation in children with type 1 diabetes mellitus (T1DM), depending on the presence or absence of diabetic complications, in order to determine the severity of endothelial dysfunction (ED).

Methods: A total of 60 children and adolescents were examined (median age 7 [3; 16] years), 30 of whom formed the main group (patients with T1DM; MG), while the other 30 were included in the control group (CG). Depending on the presence or absence of diabetic complications (neuro-, retinopathy, and nephropathy), patients from the MG were divided into two subgroups: those without complications (n=16) and those with complications (n=14). In all children, serum markers of EG degradation were determined by enzyme-linked immunosorbent assay: syndecan-1 (SDC1), syndecan-4 (SDC4), endocan-1 (EC1), heparan sulfate (HS), angiopoietin-1 (AP1), and hyaluronic acid (HA).

Results: Four out of six studied markers of EGL degradation – SDC4, HS, AP1, and HA – were elevated in the serum of patients with MG compared with children in the CG. The concentrations of SDC1 and EC1 in patients with T1DM did not differ significantly from those in the control group. The highest serum levels of SDC1, SDC4, HS, and HA were found in patients with T1DM who had diabetic complications. No statistically significant differences were found in the increase in EC1 and AP1 concentrations between children with diabetic complications and apparently healthy children.

Conclusion: High serum levels of SDC4, HS, AP1, and HA in children with T1DM indicate EG degradation and ED. Elevated concentrations of SDC1, SDC4, HS, and HA may serve as laboratory criteria for the presence of diabetic complications.

Keywords: Type 1 diabetes mellitus, children and adolescents, endothelial dysfunction, glycocalyx, diabetic complications.

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Authors' information:

Aksyonova Yulia Sergeevna,
Undergraduate Student of Stavropol State Medical University
ORCID ID: 0009-0000-0244-1377
E-mail: iu.axenowa2015@yandex.ru

Vorobyova Anna Pavlovna,
Anesthesiologist-reanimatologist, Children's City Clinical Hospital named after G.K. Filippsky
ORCID ID: 0000-0002-0082-1971
E-mail: a.v.955@yandex.ru

Bykov Yuriy Vitalievich,
Candidate of Medical Sciences, Associate Professor of the Department of Anesthesiology and Intensive Care with a Course of Additional Professorial Education, Stavropol State Medical University; Anesthesiologist-Reanimatologist, Regional Children's Clinical Hospital
ORCID ID: 0000-0003-4705-3823
E-mail: yubykov@gmail.com

Baturin Vladimir Aleksandrovich,
Doctor of Medical Sciences, Full Professor, Head of the Department of Clinical Pharmacology with a Course of Additional Professorial Education, Stavropol State Medical University
ORCID ID: 0000-0002-6892-3552
E-mail: prof.baturin@gmail.com

Mаssorov Vladislav Viktorovich,
Anesthesiologist-Reanimatologist, Children's City Clinical Hospital named after G.K. Filippsky
ORCID ID: 0009-0008-4009-1783
E-mail: Vladislav.massorov@yandex.ru

Avakyan Georgiy Arturovich,
Undergraduate Student of Stavropol State Medical University
ORCID ID: 0009-0006-7630-5734
E-mail: nepsan54@gmail.com

Information about support in the form of grants, equipment, medications

The authors did not receive financial support from manufacturers of medicines and medical equipment

Conflicts of interest: No conflict

Address for correspondence:

Bykov Yuriy Vitalievich
Candidate of Medical Sciences, Associate Professor of the Department of An- esthesiology and Intensive Care with a Course of Additional Professorial Edu- cation, Stavropol State Medical University; Anesthesiologist-Reanimatologist, Regional Children's Clinical Hospital

355031, Russian Federation, Stavropol, Mira str., 310

Теl.: +7 (962) 4430492

E-mail: yubykov@gmail.com


This work is licensed under a Creative Commons Attribution 4.0 International License.

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