Pharmacology

doi: 10.25005/2074-0581-2018-20-4-416-420
ANTIOXIDANT ACTIVITY AND CONTENT OF POLYPHENOLS IN ARTEMISIA SCOPARIA WALDST. ET KIT., GROWING UNDER THE CONDITIONS OF WESTERN PAMIR

G.Sh. Miravalova1, Sh.Sh. Kurbonbekova1, A.K. Mirzorakhimov1, S. Sattorov2, S.S. Dzhaborova3

1Institute of Botany, Physiology and Plant Genetics, Academy of Sciences of the Republic of Tajikistan, Dushanbe, Republic of Tajikistan
2Department of Microbiology and Immunology, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan
3Department of Pharmacognosy and Organization of Pharmacy Economics, Avicenna Tajik State Medical University, Dushanbe, Republic of Tajikistan

Objective: To study the antioxidant activity and polyphenol content in Artemisia scoparia Waldst. & Kit., growing in the conditions of the Western Pamir.

Methods: Materials for experiments served the plants Artemisia scoparia. Ground parts of A. scoparia collected from different heights and different slopes of the Western Pamir. Extracts were prepared from fresh leaves and stems of plants. Determination of polyphenol content performed using Folin-Ciocalteu reagents. The activity of antioxidants was determined by the ABTS method (2,2’-Azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid) analysis.

Results: Analysis of the content of polyphenols in A. scoparia, growing in the conditions of the Western Pamir, showed that it varies from 80.2 to 100.0 μg/ml. So, in Darvaz (1200 m) the content of polyphenols was 90.4 μg/ml, Rushan (1800 m) – 90.0 μg/ml, Hoigal (3025 m) – 80.2 μg/ml, Sardem (3150 m) – 90.3 μg/ml, Bijondara (2351 m) – 100.0 μg/ml. The results show some indication of the height on the concentration of polyphenols in A. scoparia. Their highest content in plants was observed in Bijondara (100.0 μg/ml) at an altitude of 2351 meters. As for the activity of antioxidants, it was found that their number decreases depending on the height of growth of plants and varies from 140 to 220 μg/ml. Their highest activity was observed in plants collected in Vanj (200.0 μg/ml) and Darvaz areas (220.2 μg/ml) at an altitude of 1220-1430 m. At an altitude of 1800 m and higher, activity gradually decreased (160,1 μg/ml), amounting to 130.7-140.1 μg/ml at an altitude of 3150 m.

Conclusions: The study showed that in the conditions of the Western Pamir, the height of growth has some influence on the accumulation of polyphenols in A. scoparia. In turn, the activity of oxidants decreases with increasing height above sea level. Perhaps this is due to the influence of stress factors of high mountains on the accumulation of biologically active substances.

Keywords: A. scoparia, polyphenols, antioxidants, a height of growth, slopes, Western Pamir.

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References
  1. Tan RX, Lu H, Wolfender JL, Yu TT, Zheng WF, Yang L, Gafner S, Hostettmann K. Mono- and sesquiterpenes and antifungal constituents from Artemisia species. Planta Med. 2009;65:64-7.
  2. Pellegrini N, Proteggente A, Pannala A, Yang M. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 2009;26:1231-7.
  3. Kaur S, Batish DR, Kohli RK, Singh HP, Mittal S. Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bioherbicide. Industrial Crops and Products. 2010;32(1):54-61. Available from: http:// dx.doi.org/10.1016/j.indcrop.2010.03.007.
  4. Ishankulova BA, Yuldasheva UP, Urunova MV. Sravnitel’naya kharakteristika nekotorykh sakharosnizhayushchikh rasteniy Tadzhikistana i antidiabeticheskikh sborov na ikh osnove [Comparative characteristics of some glucose-flowering plants and anti-diabetic collections of Tajikistan on their basis]. Vestnik Avitsenny [Avicenna Bulletin]. 2013;1:121-5.
  5. Ishankulova BA, Ismatova NS, Yuldasheva UP. Eksperimental’noe izuchenie vliyaniya ekstrakta “Gura-ob” na sekretsiyu zheludka [The experimental study of the extract «Gura-ob» influence on gastric secretion]. Vestnik Avitsenny [Avicenna Bulletin]. 2010;1:103-6.
  6. Nazarov M, Nazarov NM, Boroniev NS. Zakhirashinosii rustanihoi shifogi [Medicinal plant study]. Dushanbe, RT: 2016. p. 83-88.
  7. Filatova NS. Rod Artemisia L. Flora Tadzhikskoy SSR. T. 9. Leningrad, RF: Nauka; 1988. p. 409-468.
  8. Sharopov FS, Setzer WN. The essential oil of Artemisia scoparia from Tajikistan is dominated by phenyldiacetylenes. Natural Product Communications. 2011;6(1):119-22.
  9. Sharopov FS, Sulaymonova VA, Gulmurodov IS, Kholmadov MN, Kukaniev MA. Sostav efirnogo masla polyni metyol’chatoy (Artemisia scoparia Waldst. et Kit.), proizrastayushchey v Tadzhikistane [Composition of the essential oil of Artemisia scoparia Waldst. et Kit. growing wildly in Tajikistan]. Doklady Akademii nauk Respubliki Tadzhikistan. 2011;54(10):841-4.
  10. Singh HP, Mittal S, Kohli RK, Kaur S, Batish DR. Essential oil of Artemisia scoparia inhibits plant growth by generating reactive oxygen species and causing oxidative damage. Journal of Chemical Ecology. 2009;35(2):154-62. Available from: http://dx.doi.org/10.1007/s10886-009-9595-7.
  11. Zhigzhitzhapova SV, Randalova TJe, Radnaeva LD. Komponentnyy sostav efirnogo masla polyni metyol’chatoy (Artemisia scoparia Waldst. et Kit.), proizrastayushchey v Buryatii i Mongolii [Composition of essential oil of Artemisia scoparia Waldst. et Kit., growing in Buryatia and Mongolia]. Khimiya Rastitel’nogo Syr’ya. 2015;1:69-75. Available from: http://dx.doi. org/10.14258/jcprm.201501425.
  12. Danesh NM, Gazanchian A, Rahimizadeh M, Khayyat MH. Essential oil compositions of Artemisia scoparia Waldst. et Kit. native to North East of Iran. Advances in Environmental Biology. 2010;4(2):254-7.
  13. Zhigzhitzhapova SV, Randalova TE, Radnaeva LD. Composition of essential oil of Artemisia scoparia Waldst. et Kit. from Buryatia and Mongolia. Russian Journal of Bioorganic Chemistry. 2016;42(7):730-4.
  14. Zhigzhitzhapova SV, Dylenova EP, Radnaeva LD, Chimittsyrenov LI, Urbagarova BM, Taraskin VV. Sostav efirnogo masla polyni metyol’chatoy (Artemisia scoparia Waldst. et Kit.), proizrastayushchey v Zabaykal’e [Chemical composition of the essential oils of Artemisia Waldt. et Kit. from Trans-baikal territory]. Khimiya rastitel’nogo syr’ya. 2018;1:67-74.
  15. Zhigzhitzhapova SV, Dylenova EP, Randalova TE, Radnaeva LD, Taraskina AS. Vliyanie meteorologicheskikh usloviy na sostav efirnogo masla polyni metyol’chatoy (Artemisia scoparia Waldst. et Kit.) [The influence of the meteorological conditions on the composition of the essential oil of Artemisia scoparia Waldst. et Kit.]. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii. 2018;21(2):28-33. Available from: http://dx.doi. org/10.29296/25877313-2018-02-04.
  16. Ayrapetyan EE, Shevchuk OM, Logvinenko LA. Aminokislotnyy sostav travy polyni metyol’chatoy (Artemisia scoparia Waldst. et Kit) [Amino acid composition of herb of Artemisia scoparia (Artemisia scoparia Waldst. et Kit.)]. Vestnik Volgogradskogo gosudarstvennogo meditsinskogo universiteta. 2018;2:122-4.
  17. Kiselyova TL, Soyunova ZhA, Medvedev YuV, Chauzova AV. Fenolkarbonovye kisloty otdel’nykh predstaviteley roda polyn’ [Phenolcarboxylic acids of individual representatives of the genus Artemisia]. Farmatsevticheskaya khimiya i farmakognoziya. 2012;2:16-8.
  18. Igamberdieva PK, Oosinskaya NS. Issledovanie mineral’nogo kompleksa vegetativnoy chasti Stevia rebaundiana i Artemisia scoparia Waldst. et Kit. [The study of the mineral complex of the vegetative part of Stevia rebaundiana and Artemisia scoparia Waldst. et Kit.]. Khimiya rastitel’nogo syr’ya. 2010;4:121-4.
  19. Nazarov MN, Nazarov NM, Saidov MI, Saidov BI. Lekarstvennye rasteniya gosudarstvennoy farmakopei (Tadzhikistan) [Medicinal plants of the state pharmacopoeia (Tajikistan)]. Dushanbe, RT: Sharki Ozod; 2015. 187 p.

Authors informations:

Miravalova Gulazor Sherafgandovna, Senior Researcher of the Laboratory of Medicinal Plants, Institute of Botany, Physiology and Plant Genetics, Academy of Sciences of the Republic of Tajikistan

Kurbonbekova Shifo Shaftolievna, Candidate of Agricultural Sciences, Head of the Laboratory of Medicinal Plants, Institute of Botany, Plant Physiology and Genetics, Academy of Sciences of the Republic of Tajikistan

Mirkorakhimov Akobir Karimovich, Doctor of Biological Sciences, Leading Researcher, Laboratory of Medicinal Plants, Institute of Botany, Plant Physiology and Genetics, Academy of Sciences of the Republic of Tajikistan

Sattorov Saidbeg, Doctor of Medical Sciences, Full Professor, Head of the Department of Microbiology and Immunology, Avicenna Tajik State Medical University

Dzhaborova Sakhoba Salomudinovna, Postgraduate Student, Department of Pharmacognosy and Organization of Economics of Pharmacy, Avicenna Tajik State Medical University

Address for correspondence:

Sattorov Saidbeg

Doctor of Medical Sciences, Full Professor, Head of the Department of Microbiology and Immunology, Avicenna Tajik State Medical University

734003, Republic of Tajikistan, Dushanbe, Rudaki Ave., 139

Tel.: +992 (987) 842424

E-mail: sattoriyon@mail.ru

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