Obstetrics and Gynecology

doi: 10.25005/2074-0581-2017-19-3-286-291
HISTOLOGICAL PICTURE OF THE PLACENTAE AND FEATURES OF CD34+ EXPRESSION IN THE CELLS OF ENDOTHELIUM OF CHORION VESSELS OF PREGNANTS WITH ANAEMIA

Z.N.Salimova, M.Ya.Kamilova, D.M.Rakhmatulloeva, D.M.Gulakova

Tajik Scientific Research Institute of Obstetrics, Gynecology and Perinatology, Dushanbe, Tajikistan

Objective: To evaluate the histological features of the placentae and the degree of expression in the endothelial cells of the placental vessels of the endothelial marker CD34+ in women with anaemia and multiple births in the anamnesis.

Methods: The subject of the study was placenta of 12 women with anemia and 6 healthy women. Evaluation of endothelial marker CD34+ expression was carried out qualitatively and quantitatively using the computer image analysis system «Morphology 5.0».

Results: Patients with anaemia had expressed and moderately showed changes in the vascular bed of the chorion, regardless of the severity of the anaemia. With moderate anemia, there was chronic placental insufficiency (CPI) with a predominance of hypoplastic form. The revealed decrease in the expression of CD34+ in the placentae of women with anaemia confirms the disturbances in the functions of the vascular bed.

Conclusion: Women with anaemia are disturbed by vascular adaptation during pregnancy, leading to CPI, the intensity of which increases as anaemia worsens.

Keywords: Anaemia, placental insufficiency, histological and immunohistological studies of the placenta, endothelial marker CD34+..

Download file:


References
  1. Pulatova AP, Kamilova MYa, Mengniyazova ZG. Effektivnost’ pregravidarnoy podgotovki u zhenshchin s defitsitom mikronutrientov [Effectiveness of pregravid preparation in women with micronutrient deficiency]. Vestnik pedagogicheskogo universiteta. 2015;2:281-6.
  2. Kamilova MYa, Rakhmatulloeva DM, Ishan-Khodzhaeva FR. Meditsinskie i sotsial’nye faktory razvitiya platsentarnoy nedostatochnosti u beremennykh zhenshchin v sovremennykh usloviyakh Tadzhikistana [Medical and social risk factors for the development of placental insufficiency in pregnant women in modern conditions of Tajikistan]. Zhurnal akusherstva i zhenskikh bolezney. 2015;LXIV(6):26-30.
  3. Abdurakhmanov FM, Rasulova GT, Kamilova MYa. Prenatal’noe razvitie ploda pri beremennosti vysokogo riska [Prenatal fetal development in high-risk pregnancy]. Dushanbe, RT: Aziya-print; 2010. 159 p.
  4. Dobrokhotova YuE, Ivanova TA, Gulyaeva NV. Okislitel’nyy stress v zashchite pri fiziologicheskoy i patologicheski protekayushchey beremennosti [Oxidative stress in protection during physiological and pathologically occurring pregnancy]. Rossiyskiy vestnik akushera-ginekologa. 2008;6:33-5.
  5. Gazieva IA. Osobennosti funktsinal’nogo sostoyaniya endoteliya v pervom trimestre beremennosti v zavisimosti ot eyo iskhoda [Features of the functional state of the endothelium in the first trimester of pregnancy, depending on its outcome]. Vestnik Ural’skoy meditsinskoy akademicheskoy nauki. 2014;3:113-5.
  6. Marizoeva MM, Nematzoda O, Sultanov DD, Nazirova GO, Ismatova US, Bobdzhonova OB. Techenie beremennosti u zhenshchin s varikoznoy bolezn’yu [Course of pregnancy in women with varicose disease]. Vestnik Avitsenny [Avicenna Bulletin]. 2017;19(2):142-6. Available from: http:// dx.doi.org/10.25005/2074-0581-2017-19-2-142-146.
  7. Kamilova MYa, Rakhmatulloeva DM, Davlatova GK, Mulkamonova LN, IshanKhodzhaeva FR. Rannie i pozdnie preeclampsii: techenie beremennosti, rodov i perinatal’nye iskhody [Early and late preeclampsia: course of pregnancy, birth and perinatal outcomes]. Vestnik Avitsenny [Avicenna Bulletin]. 2016;4:34-8.
  8. Konovodova EN, Yakunina NA. Zhelezodefitsitnye sostoyaniya i beremennost’ [Iron deficiency and pregnancy]. Russkiy meditsinskiy zhurnal. 2010;19:1174-8.
  9. Burton GI, Chanoc-Jones DS, Jauniaux E. Regulation of vascular growth and function in the human placenta. Reproduction. 2009;138:895-902.
  10. Pollheimer J, Knofler M. The role of the invasive placental trophoblast in human pregnancy. Wein Med Wochenschr. 2012;162(9-10):187-90.
  11. Khuk R, Breyman K. Anemiya vo vremya beremennosti i v poslerodovom periode [Anemia during pregnancy and in the postpartum period]. Tver, RF: Triada; 2007. 74 p.
  12. Mistry HD, Gill CA, Kurlak LO. Association between maternal micronutrient status, oxidative stress, and common genetic variants in antioxidant enzymes at 15 weeks gestation in nulliparous women who subsequently develop preeclampsia. Free Radic Biol Med. 2015;78:147-55.
  13. Aylamazyan EK, Kulakov VI, Radzinskiy VE, Savelyeva GM. Akusherstvo. Natsional’noe rukovodstvo [Obstetrics. National leadership]. Moscow, RF: GEOTAR-Media; 2014. 1200 p.
  14. Kuzmin VN. Fetoplatsentarnaya nedostatochnost’: problema sovremennogo akusherstva [Fetoplacental insufficiency: the problem of modern obstetrics]. Lechashchiy vrach. 2011;3:50-4.
  15. Makarov IO. Novye vozmozhnosti lecheniya platsentarnoy nedostatochnosti [New possibilities of treating placental insufficiency]. Voprosy ginekologii, akusherstva i perinatologii. 2013;4:50-6.
  16. Shcherbina NA. Patomorfologicheskie osobennosti fetoplatsentarnogo kompleksa pri platsentarnoy nedostatochnosti i syndrome zaderzhki razvitiya ploda [Pathomorphological features of the fetoplacental complex in placental insufficiency and fetal growth restriction]. Nauchnye vedomosti. Seriya Meditsina. Farmatsiya. 2014;24(195):60-4.
  17. Nadav S, Mary DS, Rita L, Samuel P. First-trimester placental ultrasound and maternal serum markers as predictors of small-for-gestational-age infants. Am J Obstet Gynecol. 2014;211(3):253.e1–253.e8.

Author information:


Salimova Zarina Nurkhonovna,
Postgraduate Student of the Tajik Scientific Research Institute of Obstetrics, Gynecology and Perinatology

Kamilova Marhabo Yadgarovna,
Doctor of Medical Sciences, Associate Professor, Head of Obstetrics Department, Tajik Scientific Research Institute of Obstetrics, Gynecology and Perinatology

Rakhmatulloeva Dilnoza Manonovna,
Candidate of Medical Sciences, Research Fellow of the Obstetric Department, Tajik Scientific Research Institute of Obstetrics, Gynecology and Perinatology

Gulakova Dilbar Mukhamedovna,
Candidate of Medical Sciences, Research Fellow of the Obstetric Department, Tajik Scientific Research Institute of Obstetrics, Gynecology and Perinatology

Conflicts of interest: No conflict

Address for correspondence:


Kamilova Marhabo Yadgarovna

Doctor of Medical Sciences, Associate Professor, Head of Obstetrics Department, Tajik Scientific Research Institute of Obstetrics, Gynecology and Perinatology

734002, Tajikistan, Dushanbe, str. Mirzo Tursunzade, 31

Tel: (+992) 93 5009425

E-mail: marhabo1958@mail.ru

Materials on the topic: